my name is fernando sentences i am a senior at the university of arizona majoring
and physiology psychology and in spanish translation interpretation but i did my research or the
department of physiology of the college of madison
for a doctor hendrickson along with would you and process on a wrong and maybe
k time and static my research had you with a
zurich protein as the steering chinese it's called c g and terminal kind he's or
junk for short
and it had to do with its contribution to insulin resistance of them aliens skeletal
muscle
so diabetes is the main overall focus of my work and it's like you disease
that's characterized by comically elevated
fasting glucose levels in the blood insulin resistance of a million skeletal muscle is the
main defect that ultimately leads to the development of type to die one of the
ways that we know "'cause" insulin resistance is a condition cox date of stress
now octave stresses normally our body creates these things called reactive oxygen species the cell
normally has enough mechanisms to prevent them from getting too high
when they do get too high this is a condition known as ox data stress
and this figure right here shows the relationship between update of stress in the cell
the stressed activated steering traces and ultimately insulin resistance we look at as i mentioned
the p thirty eight map chinese and the gas key three
well but we haven't looked of junk yep of or what we study this is
we took healthy lena rats
and we cut out there's only a small so that the most on the back
to like we incubator the muscle in vitro and we did incubation that to four
and six hours this first crap right here i
or the glucose uptake results with no insulin hydrogen peroxide there was a significant increase
in lucas uptake but the key thing to look at is that with insulin
we have as large increase but then we have a significant drop off in we
"'cause" uptake in the muscle cell in the presence of the hydrogen peroxide and this
was characteristic along all the time points the second drop rate here is look at
the interstellar protein expression specific that is one protein call protein training to be or
at for short
and we looked at this one specifically because we know it's the it's only active
when insulin is around these two graph showed us that the issue i don't of
occidental supposed to do it decrease glucose uptake it decrease the expression of the active
form of a katie essentially indicating that some insulin resistance is occurring in the sound
well we're looking at junk specifically which is what this paragraph looked at right here
and what the c is junk expressed more the active form the fast correlated form
in the presence of hydrogen peroxide and what we found is yes that whether insulin
was present or not with the present divided in proc so they were significant increases
an active form of john all this tells us right here is that junk is
more active in the presence of a state of stress
to find of specifically what role chunk has in insulin resistance caused by oxidation stress
we had to do one separate experiment which is what this graph over here shows
us
this is a good "'cause" uptake graphical was different about this is that we incubator
the in the presence of a selective junk inhibitor the important for look at it
is red ball right here this red ball right here is a theoretical value
which we found by combining effects of each individual components this theoretical value is what
we would expect to see assuming that junk had nothing to do with insulin resistance
that we were seeing here
but in fact what we got the experiment was this part right here so this
whole difference right here is the fact that junk is having on the glucose uptake
within the muscles style and this is a significant increase in what we were expecting
to see
so what we can conclude is that the activation of this protein john which is
a searing chinese has some at least some contribution to the insulin resistance that we
see
in the million skeletal muscle cost up to date of stress and this suggests that
maybe interventions that target jong specifically could alleviate some of this insulin resistance that we
see caused by a state of stress