she is sabrina device i did research last summer at the university of washington with
each region my investigator duct "'em" you're going in my mentor was beyond
three and a bottom are
and in that lab we were interested in the function of one
put one is ubiquitous the express their of than the human body its expressed within
a red blood cells individual tissue as well as in the
what range very are also important is the fact that we don't quite understand how
time mechanism works we hypothesize that the and might be an ideal that the environment
for good one to function so we created a system using a one the yeast
cell cycle makes easier pca
which is the cell because they are you carriers like us and they should have
the proper machinery twofold
one
and we also use the week goes analog called to and beattie g and we
are using the speakers
us accuracy pca is known to uptake
tool beattie g and two in but for s as we can measure glucose uptake
we to go past minutes that already had the insert of our could gene which
was designed by a previous in turn last
summer two thousand ten i mean take that has made with are one gene and
transform it into this accuracy seriously results
i three
that when are you cells uptake our as mids be well correctly falls
and
hopefully be
be good model organism to study
to answer this question the role that live it's flea
inc one function so there were two constructs of pass minutes that we transformed into
our soccer stbc cells
one construct had a fist add
and of the constructed not have a stack the his study is there so we
can easily verify the protein it's we get a visual that the east
did express that
and the one without the first ad where how it is formed in nature
from these pictures it tell those that's accuracy vc is indeed a good model organism
to answer this question
so not that we have this study completed
we can now move on to the next which would be to and to do
the actual actually
so this task you would involve for two controls
before using
my transit unique east with the human one insert
the first control that we were gonna use is you can buy that is over
expressing collectors crewmates
and we are gonna used as a control because it
equal i
has that collections period that is known to of tape to and beattie g so
we do are passe it will be confirmation that it is working
and next we will have or a while type used to how it's not the
nature
so we can measure its androgynous
that is it's natural ability to obtain because it
we use it uses because to survive
so it does have its own
so one or two controls and then we would use our utterance unique used
to
master the glucose uptake
by our human one
so from using those two controls plus of the trends unique used that i mean
we would be able to compare the fluorescence intensity when all three cells
uptake two and we did she and so we can assess the difference in c
just to get a baseline model of the presence of the first effectively only answer
a question
would be to grow up my chance to unit keys and media containing
different types of fatty acids
and then you
and performing the same as the again
so that you can take two and beattie g so that we can see any
difference between these trends to unique used cell
and how they uptake to and beattie g
and
if we see any differences in for example we use only acid or any other
kind of fatty acid then we would be a little bit closer
understanding
the mechanism of q one function